Close the daylight plate and allow the water to spread across the sample plate. The usefulness and limitations of hand-held refractometers in The specific gravity can be measured by a. Urinometer : less accurate method. Statistical methods for assessing agreement between two methods of clinical measurement. (4, 5). Major discrepancies between biuret and refractometric results have been reported for avian samples. The validity of a method may be determined by comparing it with another method known to be valid.23, To our knowledge, this is the first study to examine both the reliability and validity of methods commonly used to measure urine specific gravity. official website and that any information you provide is encrypted Disadvantages Of Refractometer - 1672 Words | Bartleby HHS Vulnerability Disclosure, Help Calibrate the refractometer by placing distilled water on the glass as the sample, 3 Measurement. The dotted line represents zero difference between the 2 methods and the 2 solid lines 2 standard deviations of the difference between methods. In a routine urinalysis, the urine is checked for its appearance, concentration, and composition. It works by measuring the refraction of light through the liquid and calculating the sugar content based on that measurement. The refractometer and hydrometer were reliable between trials, but the reagent strips were not. (3) For the determination of specific gravity of canine urine the urinometer is often used. WebDensity of solution and density of same amount of distilled water are compared at same temperature. The validity of hydrometry and reagent strips was assessed by comparison with refractometry, the criterion measure for urine specific gravity. Choose page types: Centre for the History of Medicine, Humanities Building room 449a, University of Warwick, Coventry CV4 7AL Medical hand-held refractometers have been used in veterinary practice since their development in the 1960s. Bland JM, Altman DG. (4) Definition. Numerous authors have reported that the results of protein estimation by refractometry for domestic mammals correlate well with those obtained by the biuret method, although others have reported both higher and lower refractometric results compared with biuret results. Compared to the urinometer, it offers several advantages: only 1-2 drops of urine suffice for the examination; accurate compared to the specific gravity and therefore favored. Image 1: Urinalysis is a part of the routine check-up. Urine samples were divided into 2 subsamples (trials A and B) for analysis. The strength of the intraclass correlation coefficients was assessed according to the rating scale of Vincent.23 We prepared Bland-Altman plots21 to evaluate agreement between measurement methods for urine specific gravity. The sensitivity of a test indicates how well a test finds disease positives (ie, those with the condition, such as dehydration).
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