Before Google Scholar, Gilman AC, Rall TW, Nies AS, Tayor P (2001) Goodman and Gilmans the pharmacological basis of therapeutics, 10th edn. Shukla D, Konar S, Devi BI, Padmasri G, Jayanna R, Suresh M, Lakshita B. And by substituting in the above regression equation previously formulated postulated amplitude of CAF alone at 263.6nm (iso). Correspondence to The amplitudes difference of the obtained ratio spectrum at 263.6nm (iso) and 240nm were recorded (Pm). Talanta 75:258265, El-Gindy A, Emara S, Shaaban H (2010) Validation and application of chemometrics-assisted spectrophotometry and liquid chromatography for simultaneous determination of two ternary mixtures containing drotaverine hydrochloride. Then derivative of these ratio spectra was obtained to remove the constant generated of PCT concentration in the division spectrum. Biomed Res Int. The concentration of PCT was progressively determined by subtraction of the obtained CAF concentration from the total concentration at isosbestic point (iso 263.6nm) recorded before derivatization. et al. sharing sensitive information, make sure youre on a federal Abstract. For the determination of concentration of component of interest by the DD-RD-DW method, the component of interest shows a significant amplitude difference at two selected wavelengths 1 and 2 where the two interfering substances used as double divisor give constant amplitude as while the third one shows the same amplitude values at these two selected wavelengths. HML put the theoretical background, revised the manuscript, revised the results, and collected the data. Intracerebral hematomas are detectable to a maximum depth of 2.5 cm, depending on thickness and diameter. Three different novel, simple and accurate methods were adopted for simultaneous determination of CAF and PCT in presence of each other either in bulk, in different dosage forms as binary mixture and in presence of other components after their resolutions. Please enable it to take advantage of the complete set of features! The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. The proposed methods also showed the significance of isoabsorptive point, normalized spectra as divisors and dual wavelengths as powerful tools that could either be used alone or in combination with each other for the resolution of severely overlapped spectra without preliminary separation. Two distinguishable light emitting sources (810 nm and 1300 nm) are utilized at isosbestic points with a single photodetector (800-1700 nm).
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